{"id":14529,"date":"2018-04-03T04:16:34","date_gmt":"2018-04-03T04:16:34","guid":{"rendered":"http:\/\/attekno.com\/?page_id=14529"},"modified":"2024-08-14T21:40:39","modified_gmt":"2024-08-14T14:40:39","slug":"fermentor","status":"publish","type":"page","link":"https:\/\/attekno.com\/index.php\/scientific\/fermentor\/","title":{"rendered":"MINIFOR Laboratory Fermentor\u2013Bioreactor"},"content":{"rendered":"

Fermentor – Bioreactor<\/span><\/h2>\n
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DESCRIPTION<\/b><\/strong><\/p>\n

ONE INSTRUMENT FOR:<\/b><\/strong><\/p>\n

– High density microbial fermentation (Bacteria, Yeast, Fungi)
\n– Batch\/Fed-batch\/Perfusion\/Continuous cultures
\n– Aerobic \/ anaerobic fermentation
\n– Protein \/ Vaccine \/ Monoclonal antibody production
\n– Animal \/ Mammalian \/ Insect cell culture
\n– Controlled Stem cell culture
\n– Tissue Engineering
\n– Algal \/ Plant cell culture
\n– Universities \/ Life sciences & technical Schools<\/p>\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n
Technical specification<\/b><\/strong>\u00a0<\/b><\/strong><\/p>\n

\u00a0<\/b><\/strong><\/p>\n

Power <\/b><\/strong><\/td>\n

Universal power supply for mains 100-245 V AC\/50-60Hz, 560W, CE conform<\/td>\n<\/tr>\n
Display <\/b><\/strong><\/td>\nLCD 4 x 40 digits with backlight illumination<\/td>\n<\/tr>\n
Dimensions <\/b><\/strong><\/td>\n22 x 40 x 38 cm (W x D x H)<\/td>\n<\/tr>\n
Fermentor vessel <\/b><\/strong><\/td>\nPyrex borosilicate glass with 6 to 8 threaded necks; 0.3, 0.4, 1, 3, 7 liter vessels<\/td>\n<\/tr>\n
Temperature control <\/b><\/strong><\/td>\nHigh efficiency 150 W infrared (IR) radiation heat source with gilded parabolic reflector<\/td>\n<\/tr>\n
Regulation<\/td>\nFrom 5\u00b0C over RT to 70\u00b0C<\/td>\n<\/tr>\n
Measurement<\/td>\nFrom 0 to 99.9\u00b0C in 0.1\u00b0C steps<\/td>\n<\/tr>\n
Precision<\/td>\n+\/- 0.2\u00b0C (0 to 60\u00b0C)<\/td>\n<\/tr>\n
Sensor<\/td>\nPt 100 incorporated in the glass electrode of the pH probe<\/td>\n<\/tr>\n
pH control <\/b><\/strong><\/td>\nSterilizable pH electrode pH 0-14 with automatic temperature correction, two-point semi-automatic calibration and Variopin connector<\/td>\n<\/tr>\n
Resolution<\/td>\n0.01 pH unit<\/td>\n<\/tr>\n
Precision<\/td>\n+\/- 0.02 pH unit<\/td>\n<\/tr>\n
pO2 control <\/b><\/strong><\/td>\nSterilizable Clark type oxygen sensor with fast response, automatic temperature correction, two-point semiautomatic calibration, dissolved oxygen (DO) control through regulation of the airflow rate<\/td>\n<\/tr>\n
Range<\/td>\n0 to 25 mg oxygen\/ l, in 0.1 mg\/l steps<\/td>\n<\/tr>\n
Air flow <\/b><\/strong><\/td>\n0 to 5 l\/min in 0.01 l\/min steps, measured by precise mass flow meter, linearity +\/- 3%, reproducibility +\/- 0.5%<\/td>\n<\/tr>\n
Control<\/td>\nProportional valve controlled by microprocessor<\/td>\n<\/tr>\n
Supplied air pressure<\/td>\n0.05 \u2013 0.2 MPa (0.5 – 2 atm)<\/td>\n<\/tr>\n
Agitation <\/b><\/strong><\/td>\n50 W Vibromixer 0 to 20 Hz (0 to 1200 rpm) in 0.1 Hz steps (6 rpm) with 1 or more stirring discs; Sterility similar to magnetic coupling<\/td>\n<\/tr>\n
Selectable parameter <\/b><\/strong><\/td>\nAn additional parameter can be controlled by the instrument (foaming control, weight (for continuous cultures), pCO2, redox potential, conductivity, optical density, etc.); with standard 0-10V or 0-20mA output<\/td>\n<\/tr>\n
Ports <\/b><\/strong><\/td>\nOne large quadruple sampling or additions port with four needles with LAMBDA PEEK double-seal connections, used for sampling, inoculation, antifoam, feeds, harvest, addition of correction solutions etc., additional double ports are available<\/td>\n<\/tr>\n
Pumps <\/b><\/strong><\/td>\nUp to 4 independent pumps (PRECIFLOW, MULTIFLOW, HIFLOW or MAXIFLOW) with speed variation from 0 to 100 % can be used with MINIFOR laboratory fermenter-bioreactor<\/td>\n<\/tr>\n
Gas flow control <\/b><\/strong><\/td>\nIn addition to pumps, several electronic flow controllers with flow rate ranges of 0-5 l\/min (MASSFLOW 5000) or 0-500 ml\/min (MASSFLOW 500) can be used for the controlled addition of gases (e.g. N2, O2, air, CO2) in cell cultures; freely configurable gas station module<\/td>\n<\/tr>\n
Working temperature <\/b><\/strong><\/td>\n0 – 40 \u00b0C<\/td>\n<\/tr>\n
Working humidity <\/b><\/strong><\/td>\n0 – 90 % RH, not condensing<\/td>\n<\/tr>\n
Security <\/b><\/strong><\/td>\nIEC 1010\/1<\/td>\n<\/tr>\n
Weight <\/b><\/strong><\/td>\n7.5 kg<\/td>\n<\/tr>\n
PC control <\/b><\/strong><\/td>\nComplete PC control and data processing using the fermentation software FNet (for up to 6 MINIFOR fermenters) or SIAM (for an even higher number of instruments)<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n
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Through innovation to quality lab scale fermenter\/fermentor and bench-top bioreactor at half-cost. New concepts in laboratory fermentation and cell cultures:<\/p>\n<\/div>\n<\/div>\n

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MINIFOR laboratory fermentor and bioreactor<\/h2>\n

The LAMBDA MINIFOR bench-top autoclavable laboratory fermenter and bioreactor was developed as a result of the need to construct a small laboratory fermentor for volumes from\u00a035 ml to over 6 liters<\/strong>.<\/p>\n

Based on long personal practical experience of fermentations, a fermentor-bioreactor which is easy to use and with the capacity to\u00a0measure and control all the important parameters<\/strong>\u00a0of the biological culture, has been created.\u00a0This required\u00a0new ideas and many innovations.<\/p>\n

\"LAMBDA<\/p>\n

Smallest footprint for parallel experiments<\/h2>\n

The fermentor had to take up\u00a0minimum space on the bench<\/strong>\u00a0but with\u00a0good access to all parts<\/strong>. Several fermentors should when placed side by side be suitable for the optimization of the parameters of growth of culture or optimization of bio-transformations etc. (highly suitable for\u00a0parallel running experiments).<\/p>\n

The space requirement of the MINIFOR fermentor-bioreactor with working vessel and the control unit is: 22 cm x 38 cm x 40 cm (W x H x D).<\/p>\n

\"Compact<\/p>\n

Bioreactor operation, remote control and data acquisition<\/h2>\n

LAMBDA MINIFOR fermentor and bioreactor\u00a0can be fully controlled and operated from the front panel<\/strong>. All parameters can be immediately seen without scrolling and set values as well as high and low alarms of each parameter can be adjusted\u00a0on the front panel.<\/p>\n

Several MINIFOR units can be connected to one PC and the\u00a0optional fermentation software SIAM or FNet<\/strong>, allows remote control and data processing. For connecting multiple MINIFOR fermenter-bioreactor units to the PC,\u00a0no additional software licenses needed<\/strong>.<\/p>\n

Innovations in MINIFOR fermentor-bioreactor for excellent price to performance ratio<\/h2>\n

To keep the cost of the MINIFOR fermentor-bioreactor low, without compromising quality, several new ideas and innovations have been introduced:<\/strong><\/p>\n

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  • Instead of a fermentor flask with a\u00a0stainless steel head plates, which is expensive, we use\u00a0whole autoclavable glass vessels\u00a0<\/strong>(0.3 L, 0.4 L, 1 L, 3 L and 7 L)<\/em>\u00a0with threaded fittings. They have been used for many years in cell culture and are proved to\u00a0maintain perfect sterility<\/strong>. Thanks to this concept, the MINIFOR fermenter-bioreactor is set up in shortest possible time.
    \n\"MINIFOR<\/li>\n
  • Instead of a traditional propeller agitator, which requires an expensive motor and magnetic coupling, we have introduced a new\u00a0up-and-down agitation<\/strong>. A motor together with an inexpensive membrane perfectly assure sterility and produce an\u00a0efficient mixing<\/strong>\u00a0without formation of a vortex\u00a0(no baffles needed)<\/strong>. At the same time this type of\u00a0mixing is gentler on cells<\/strong>\u00a0and\u00a0produces less foam<\/strong>. Novel\u00a0biomimicking \u201cfish-tail\u201d stirring discs\u00a0offer\u00a0maximum mixing efficiency without cutting edges<\/strong>.<\/li>\n
  • The culture is heated by heat radiation produced in a\u00a0parabolic radiator with a gold reflector placed under the fermentation vessel. The heat is absorbed gently in the culture similarly to the sun heating water. There is\u00a0no overheating of the culture at any volume<\/strong>\u00a0and expensive double-wall vessels with thermostatic baths are eliminated. At the same time tubing and cables disappear making the fermentor\u00a0less complex<\/strong>.<\/li>\n
  • As far as possible\u00a0expensive pieces of equipment have been replaced<\/strong>\u00a0by new high performance plastics.<\/li>\n
  • By using modern microprocessors it has been possible to place all the electronics in the front part of the instrument. This makes the fermentor\u00a0unbelievably compact\u00a0<\/strong>and\u00a0eliminates the casing tower<\/strong>. Despite its small size\u00a0six parameters are measured and controlled<\/strong>\u00a0in the basic configuration of the MINIFOR.<\/li>\n<\/ul>\n<\/div>\n<\/div>\n<\/div>\n
    <\/div>\n","protected":false},"excerpt":{"rendered":"

    Fermentor – Bioreactor DESCRIPTION ONE INSTRUMENT FOR: – High density microbial fermentation (Bacteria, Yeast, Fungi) – Batch\/Fed-batch\/Perfusion\/Continuous cultures – Aerobic \/ anaerobic fermentation – Protein \/ Vaccine \/ Monoclonal antibody production – Animal \/ Mammalian \/ Insect cell culture – Controlled Stem cell culture – Tissue Engineering – Algal \/ Plant cell culture – Universities \/ Life sciences & technical Schools Technical specification\u00a0 \u00a0 Power… <\/p>\n","protected":false},"author":1,"featured_media":0,"parent":14424,"menu_order":0,"comment_status":"closed","ping_status":"closed","template":"","meta":{"_joinchat":[],"footnotes":""},"class_list":["post-14529","page","type-page","status-publish","hentry","clearfix"],"_links":{"self":[{"href":"https:\/\/attekno.com\/index.php\/wp-json\/wp\/v2\/pages\/14529","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/attekno.com\/index.php\/wp-json\/wp\/v2\/pages"}],"about":[{"href":"https:\/\/attekno.com\/index.php\/wp-json\/wp\/v2\/types\/page"}],"author":[{"embeddable":true,"href":"https:\/\/attekno.com\/index.php\/wp-json\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"https:\/\/attekno.com\/index.php\/wp-json\/wp\/v2\/comments?post=14529"}],"version-history":[{"count":14,"href":"https:\/\/attekno.com\/index.php\/wp-json\/wp\/v2\/pages\/14529\/revisions"}],"predecessor-version":[{"id":19385,"href":"https:\/\/attekno.com\/index.php\/wp-json\/wp\/v2\/pages\/14529\/revisions\/19385"}],"up":[{"embeddable":true,"href":"https:\/\/attekno.com\/index.php\/wp-json\/wp\/v2\/pages\/14424"}],"wp:attachment":[{"href":"https:\/\/attekno.com\/index.php\/wp-json\/wp\/v2\/media?parent=14529"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}