The falling pad method increases the transfer efficiency as gravity promotes the transfer of the buffer in addition to water absorption by “Pad” and paper towel.
Applications
•Transfer in Southern blotting
•Transfer in Northern blotting
Features
Works with a smaller amount of buffer than the conventional one
The efficiency of the falling pad method and the effect of the water
absorption pad reduces the buffer amount.
The effective transfer in just 2 hours. Even DNA/RNA with a large molecular amount.
Conventionally, the membrane was placed on the gel, and the paper towel transferred the band to the membrane with the force of sucking up the buffer. In other words, it had defied gravity. This product was able to obtain the synergy effect and prompt transfer by adding gravity to the capillary force of the paper towel to buffer by placing the membrane under the gel. Smooth transfer of even DNA/RNA with a large molecular amount (See next page for the structure).
The Resin water absorption pad can be used for many times
The Resin water absorption pad is adopted to reduce the amount of
paper towel used. It can be used for many times by washing with water
and naturally drying after use.
The structure of G Capillary blotter
As the gel is placed on the membrane and the water absorption
pad is placed under the gel, the buffer flows down by gravity
and absorption. Accordingly, the DNA and RNA in the gel are
transferred to the membrane. Since the DNA and RNA cannot
pass through the membrane, they are trapped on the membrane.
The transfer continues as the buffer continues to flow downward
by the absorbent pad.
Specifications
| Model | G Capillary Blotter D-set |
|---|---|
| Max. Gel size | 120 x 120mm |
| Configuration | Pad bath PB-2426 (Base part 350 x 220 mm, Bath inner 290 x 200 x 40Hmm) Buffer bath (upper part) BF-2426 Pad P-1515 (150 x 150 mm) |
| •If the new pads do not absorb enough water, soak them in the buffer for a while before use. Wash the pads with water and allow it to dry naturally after use (The water absorption decreases if it is put in the dryer, etc.) |